SCHEDULE
Friday, Nov 25th
Saturday, Nov 26th
Sunday, Nov 27th
8.00-8.45 : Registration
8.45-9.00 : Open ceremony (MY)
Taking group Picture
9.00-10.00 : Lecture FR-1
10.00-10.15 : Coffee break [Block (C) by PS]
10.15-11.15 : Lecture MB-1
11.15-11.30 : Laboratory Brief (MY)
11.30-12.30 : Wash and Add scFv-AP (C)
Innoculate cells (D)
12.30-13.30 : Lunch
13.30-14.30 : Wash and Add AP substrate (C)
14.30-14.45 : Coffee break
14.45-15.45 : Measure OD 405 nm (C)
Add IPTG (D)
Pick colonies (B)
16.00-17.00 : Immobilized target (A)
Collect Cells (D)
Pick TG1 (A) [by TS]
19.00-22.00 : Welcome party
9.00-10.00 : Lecture FR-2
10.00-10.15 : Coffee break [Block by staff; (A)]
10.15-11.15 : Lecture MB-2
11.15-11.30 : Laboratory brief
11.30-12.00 : Dilute 1:100 infected E.coli (B)
Dilute 1:100 E.coli (A) [by NP]
Add library (A)
Measure OD 405 nm , [ovn] (C)
Break Cells (D)
12.00-13.00 : Lunch
13.00-14.00 : SDS-PAGE & FPLC (D)
14.00-15.00 : Add helper phage (B)
15.00-15.30 : Wash and Elute phage (A)
Keep TG1 on ice (A)[by NP]
Infect eluted phage into TG1 (A)
15.30-16.00 : Immobilized target for ELISA (B) [by NP]
16.00-17.00 : Centrifuge and change media (B)
Spread plate (A)
9.00-10.00 : Lecture FR-3
10.00-10.15 : Coffee break
[Block and centrifuge phage by staff; (B)]
[Collect plate by staff; (A)]
10.15-11.15 : Lecture MB-3
11.15-11.30 : Laboratory brief
11.30-12.00 : Wash and add supernatant (B)
12.00-14.00 : Lunch Buffet & Discussion
13.00-14.00 : Wash and add anti-M13 (B)
15.00-16.00 : Wash and add ABTS substrate (B)
16.00: Closing ceremony
Certificate Presentation
DAY A. Biopanning B. Phage ELISA C. ELISA (AP) D. Expression
(Kun and lady Kik) (Aom and Thae thae) (Fang) (Bee)
----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
F 25/11/16 Coat target Pick colonies ELISA Break cell
(Wash by machine)
S 26/11/16 Biopanning Phage amplification (Purify by machine)
(YAMO) * Optional*
Su 27/11/16 Collect colonies Phage ELISA SDS-PAGE
(Wash by bottle)
LECTURES
FR
FR-1 : Protein Libraries : Design & Construction
FR-2 : Surface Display Technologies
FR-3 : Fc Antibody Engineering
MB
MB-1 : Disulfide bond biology and protein expression
MB-2 : SHuffle expression system
MB-3 : SHuffled expressed antibody